培育方式对双胞胎湖羊羔羊肝基因表达的影响

doi:10.11843/j.issn.0366-6964.2016.04.013

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (4): 733-744.doi: 10.11843/j.issn.0366-6964.2016.04.013

培育方式对双胞胎湖羊羔羊肝基因表达的影响

王海超，张乃锋，柴建民，王波，刁其玉^*

（中国农业科学院饲料研究所农业部饲料生物技术重点实验室，北京 100081）

收稿日期:2015-05-05 出版日期:2016-04-23 发布日期:2016-04-23
通讯作者: 刁其玉，研究员，博士生导师，E-mail：diaoqiyu@caas.cn
作者简介:王海超（1990-），男，河北临漳人，硕士生，主要从事反刍动物营养学研究，E-mail：qiusuoba@163.com
基金资助:
农业部公益性行业（农业）科研专项（201303143）；国家肉羊产业技术体系（CARS-39）

Effect of Different Rearing Systems on Hepatic Gene Expression of Early Weaned Hu Twin Lambs

WANG Hai-chao，ZHANG Nai-feng，CHAI Jian-min，WANG Bo，DIAO Qi-yu^*

(Key Laboratory of Feed Biotechnology of Ministry of Agriculture，Feed Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China)

Received:2015-05-05 Online:2016-04-23 Published:2016-04-23

摘要/Abstract

摘要：

旨在研究培育方式对双胞胎湖羊羔羊肝基因表达的影响。选取24对双胞胎湖羊公羔羊，采用配对试验设计，将每对双胞胎羔羊均匀分成两组，一组为试验组，7日龄后实行人工哺育代乳粉(Artificially reared，AR)；另一组为对照组，进行随母哺乳(Ewe reared，ER），每组24只羔羊。两组羔羊均在60日龄断奶，之后饲喂开食料至90日龄。羔羊在60和90日龄时，分别随机选取3对双胞胎羔羊进行屠宰。用基因芯片技术和生物信息学的方法分析肝组织的差异表达基因。通过基因芯片分析，本试验发现了许多差异表达基因。AR组和ER组间差异表达基因在60和90日龄都发生改变的基因有7个，它们是OR1E2、S1PR3、KMO、APOA4、ORM2、HTR4和FADS1；60和90日龄间的差异表达基因在AR组和ER组中表达也发生变化的基因有20个，它们是ACO1、HBA1、MFSD2A、CYSJ、LOC785954、FCN、GRB2、LOC100037663、ORM2、RXRG、LOC100602447、SQLE、HSD17B2、CYP4F2、INHBE、EAAT2，其中有4个未知基因，探针号为14780690、14815720、14845504、14854080；在不同日龄和不同处理中，表达均发生改变的基因是ORM2。GO功能显著性富集分析发现，这些差异表达基因主要参与氨基酸、小分子化合物和有机酸代谢的生物过程、酶活性反应生物过程和免疫反应过程。KEGG代谢途径分析发现，这些差异表达基因主要参与氨基酸代谢、脂类代谢、激素合成和信号转导途径。综上所述，不同的培育方式导致肝的基因表达发生了改变，发现了肝差异表达基因参与的生物学过程及其发挥的生物学功能，初步揭示了羔羊早期培育的分子机理。

Abstract:

This experiment was conducted to study the effect of different rearing systems on hepatic gene expression in twin lambs.Twenty-four pairs of Hu male twin lambs were equally divided into 2 groups.Twenty-four subjects were artificially reared(AR) with milk replacer after 7 days colostrum while 24 others were ewe reared(ER) with mother milk as control.All lambs were weaned at day 60 and then fed with starter till day 90.When the lambs reached 60 and 90 days old，three pairs of twin lambs at each time point were slaughter to collect liver samples.Hepatic gene expression were analyzed via microarray and bioinformatics method.We found many differentially expressed genes(DEGs).They were OR1E2，S1PR3，KMO，APOA4，ORM2，HTR4，FADS1 in both 60AR vs 60ER and 90AR vs 90ER，and ACO1，HBA1，MFSD2A，CYSJ，LOC785954，FCN，GRB2，LOC100037663，ORM2，RXRG，LOC100602447，SQLE，HSD17B2，CYP4F2，INHBE，EAAT2 in both 60AR vs 90AR and 60ER vs 90ER，among which 4 unknown genes were identified with the probe ID 14780690，14815720，14845504，14854080.ORM2 was differentially expressed among all the comparisons.Those DEGs involved in the amino acid metabolism，small molecular metabolism，enzyme activities and immune response of GO process and amino acid metabolism，lipid metabolism，hormone biosynthesis and signaling transductions of KEGG pathways.In summary，different rearing systems altered hepatic gene expression.The pathways and functions those DEGs involved in could shed lights on the mechanisms of early cultivation of lambs.

中图分类号:

S826
S815.4

王海超，张乃锋，柴建民，王波，刁其玉. 培育方式对双胞胎湖羊羔羊肝基因表达的影响[J]. 畜牧兽医学报, 2016, 47(4): 733-744.

WANG Hai-chao，ZHANG Nai-feng，CHAI Jian-min，WANG Bo，DIAO Qi-yu. Effect of Different Rearing Systems on Hepatic Gene Expression of Early Weaned Hu Twin Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 733-744.

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培育方式对双胞胎湖羊羔羊肝基因表达的影响

Effect of Different Rearing Systems on Hepatic Gene Expression of Early Weaned Hu Twin Lambs

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